The application of mass spectrometry to food analysis

Caprioli, Giovanni (2011) The application of mass spectrometry to food analysis. PhD thesis, SCHOOL OF ADVANCED STUDIES - Doctorate course in Pharmaceutical sciences (XXIII cycle).

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Mass spectrometry (MS) is an analytical technique that measures the molecular masses of individual compounds and atoms precisely by converting them into charged ions. For over 100 years, it has played a pivotal role in a variety of scientific disciplines. With a small beginning in the late nineteenth century as a tool to detect cathode rays, mass spectrometry currently has become an integral part of proteomics and drug development process. Several diverse fields, such as physics, chemistry, medicinal chemistry, pharmaceutical science, geology, nuclear science, archeology, petroleum industry, forensic science, and environmental science, have benefited from this highly sensitive and specific instrumental technique. In my thesis, Mass Spectrometry has been applied in food chemistry fields for the analysis of toxic compounds and contaminants, for nutraceutics and for the characterization of foodstuff to be applied for production areas and traceability. In the first part, high resolution mass spectrometry (LTQ-Orbitrap) has been used for the structural characterization and quantification of toxic compounds (glycoalkaloids and aglycons) in potato, tomato and potato upper soil. The second part of the study has been related to the analysis of lentils by using two different mass analyzers: TOF and single quadrupole. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS with TOF mass spectrometer) was used in this study to obtain protein fingerprints of seven different lentil varieties, to characterize their differences and similarities. With single quadrupole, we developed a new analytical procedure for quantification of the content of two cholesterol lowering compounds, soyasaponin I and VI, in various cultivars of Italian lentils, by SPE-HPLC-MS. The third part of the study the analysis of ink photoinitiators in policoupled carton packaging and in contained food beverages. A new analytical method that uses gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-mass spectrometry (LC/MS) was developed for the analysis of five photoinitiators. Different mass analyzers were used for the quantification of 40 real samples,which resulted more or less contaminated. LC-APPI/MS/MS was used to confirm the obtained results on benzophenone contamination, showing the importance both of the ionization source and of the tandem mass spectrometry in the analysis of trace contaminants. Finally, a comparison of the performance characteristic of the used mass spectrometers was reported.

Item Type:Tesi (PhD)
Informazioni aggiuntive:Tutor: Prof. Sauro Vittori; Dott. Gianni Sagratini
Settori scientifico-disciplinari:SCIENZE CHIMICHE > CHIM/10 Chimica degli alimenti
ID Code:242
Depositato da:Doctor Giovanni Caprioli
Depositato su:24 Jan 2011 09:23
Last Modified:25 Feb 2014 15:24

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